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Publication numberCN102550452 B
Publication typeGrant
Application numberCN 201110458867
Publication date11 Sep 2013
Filing date31 Dec 2011
Priority date31 Dec 2011
Also published asCN102550452A
Publication number201110458867.8, CN 102550452 B, CN 102550452B, CN 201110458867, CN-B-102550452, CN102550452 B, CN102550452B, CN201110458867, CN201110458867.8
Inventors杨海萍, 吴建平, 刘海林, 张志勇, 沈德华, 许津, 徐献民
Applicant江苏省海洋水产研究所
Export CitationBiBTeX, EndNote, RefMan
External Links: SIPO, Espacenet
Cultivating method of Sea anemone parents
CN 102550452 B
Abstract  translated from Chinese
本发明涉及一种海葵亲体培养方法,包括:采集健康、活力强、无损伤、触手齐全,足部完好的海葵,置于亲体培养系统中培养,使之成熟产卵、排精,配子受精后收集受精卵,移入育苗池中进行胚胎培育和育苗。 The present invention relates to a method of anemone broodstock culture, including: collection of health, vitality and strong, no damage, tentacle complete, intact foot anemone, placed broodstock culture system culture, so that mature eggs, ejaculation, gamete After fertilization fertilized eggs are collected and transferred to breeding ponds and nursery nurture embryonic. 本发明的海葵亲体培养方法具有方便投喂管理,便于长期培养,易于观察,培养效果好,水体循环利用,受精卵连续收集等优点,解决了挖掘生活型海葵沉性受精卵难以有效收集的技术难点,受精卵孵化率高,残饵及消化残余物易清除,可操作性强,具有良好的实用性。 Anemone broodstock culture method of the present invention has a convenient feeding management, facilitate long-term culture, easy to observe, train effect, water recycling, the fertilized egg is continuously collected, etc., to solve the mining life form anemones sinking difficult to effectively collect the fertilized egg technical difficulties, hatching rate, bait and digestion residues easy to clean, easy to operate, with good usability.
Claims(7)  translated from Chinese
1.一种海葵亲体培养方法,包括以下步骤: A.亲体采集: 采集海葵亲体,准备亲体培养; B.建立亲体培养系统,包括: 亲体培养槽(1),培养架(2),亲体培养罐(3),出水管(5),残饵及消化残余物分离槽(6),集卵网箱(7),集卵槽(8),进水管(9),海葵亲体(11),散气石(12),气泵及气路(13),水泵及管路(14),其中:海葵亲体(11)培养于亲体培养罐(3)中,亲体培养罐(3)置于培养架(2)中,培养架(2)安放于亲体培养槽(I)中,水流通过出水管(5)流入残饵及消化残余物分离槽(6),受精卵随水流入集卵网箱(7),集卵网箱(7)置于集卵槽(8)中,水体经水泵及管路(14)、进水管(9)流入亲体培养槽,气泵及气路(13)连接散气石(12),给散气石(12)供气; 步骤B所述系统中亲体培养槽(I)为圆形,中央底部为平缓的锥形,培养槽(I)中央设亲体阻隔筒(4),中央水流(17)通过亲体阻隔筒(4)经中央出水口(16)流入出水管(5),散气石(12)在亲体阻隔筒(4)内产生气升作用使亲体培养槽(I)中形成垂直对流(15),进水管(9)沿亲体培养槽(I)上口切线方向设置,水泵及管路(14)使进水管(9)造成射流形成水平环流(18)。 A sea anemone broodstock culture method comprising the following steps: A. broodstock collection: anemone broodstock collection, preparation broodstock culture; B. establish broodstock culture systems, including: broodstock fermentation tank (1), training aircraft (2), broodstock culture tank (3), the outlet pipe (5), bait and digestion residue separation tank (6), collect eggs cages (7), egg collection groove (8), inlet (9), sea anemones broodstock ( 11), bulk air stone (12), air pump and air passage (13), pumps and piping (14), wherein: anemone parent body (11) were cultured in broodstock culture tank (3), the parent body culture tank (3) placed training aircraft (2) training aircraft (2) placed in with the water flowing into the culture vessel set broodstock (I), the water flows into the bait and digested residue separation tank (6) through the outlet pipe (5), the fertilized egg egg cages (7), set egg cages (7) in egg collection groove (8), the water by the pump and piping (14), inlet pipe (9) into the parent body of the fermentation tank, pump and gas path (13 ) stone bulk gas connection (12), to loose air stone (12) gas; step system in the B broodstock culture tank (I) is circular, the central bottom gently tapered, culture tank (I) center set up broodstock barrier tube (4), the central stream (17) barrier tube through broodstock (4) by the central outlet (16) into the outlet pipe (5), bulk air stone (12) blocking cylinder in broodstock production (4) Airlift the role of a parental culture tank (I) formed in the vertical convection (15), inlet (9) along broodstock culture tank (I) catchy tangential direction, pumps and pipes (14) of the intake pipe (9) resulting jet formation horizontal circulation (18).
2.根据权利要求1所述的一种海葵亲体培养方法,其特征在于:所述的海葵亲体是中华仙影海葵亲体。 2. An anemone claim 1, wherein the pro-culture method, wherein: the anemone parent body is the Chinese Xian Ying anemone broodstock.
3.根据权利要求`1所述的一种海葵亲体培养方法,其特征在于:步骤A采集的海葵亲体是在3-4月采集的健康、活力强、无损伤、触手齐全、足部完好的海葵亲体。 `According to claim 1, wherein the parent of a sea anemone culture method, wherein the steps of: A collection of sea anemones broodstock in March-April acquisition of healthy, vibrant and strong, no damage, tentacle complete, foot anemone intact broodstock.
4.根据权利要求1至3中任一所述的一种海葵亲体培养方法,其特征在于:步骤B所述系统中还包括环形培养架模块(19)和扇形培养架模块(20),其中I个环形培养架模块(19)和多个扇形培养架模块(20 )组合成培养架(2 )整体安放于亲体培养槽(I)内,多个亲体培养罐(3)均匀安装于培养架(2)上,调节水泵及管路(14)流速保持水位(10)。 1-3 according to any one of the parent of a sea anemone culture method claims, characterized in that: the B step system also includes an annular training aircraft module (19) and fan culture frame module (20), which I annular training aircraft module (19) and a plurality of fan culture rack module (20) into a training aircraft (2) as a whole placed in broodstock culture tank (I) within a plurality of broodstock culture tank (3) mounted on a uniform culture frame (2), adjust the pump and piping (14) maintaining the flow rate level (10).
5.根据权利要求4所述的一种海葵亲体培养方法,其特征在于:其中I个环形培养架模块(19)和8个扇形培养架模块(20)组合成培养架(2)整体安放于亲体培养槽(I)内,160个亲体培养罐(3)均匀安装于培养架(2)上。 5. An anemone broodstock culture method according to claim 4, wherein: where I annular training aircraft module (19) and eight sector training aircraft module (20) into a training aircraft (2) Overall place in broodstock culture tank (I) within, 160 broodstock culture tank (3) uniformly attached to the training aircraft (2).
6.根据权利要求1至3中任一所述的一种海葵亲体培养方法,其特征在于:人工投喂海葵饵料供海葵亲体(11)摄食,残饵及消化残余物随水流进入残饵及消化残余物分离槽(6)。 1 according to any one of the three pro-anemone a culture method of claim wherein: artificial feeding bait for sea anemone anemone parent body (11) feeding, bait and digest the residue with the water into the bait and digestion residue separation tank (6).
7.根据权利要求1至3中任一所述的一种海葵亲体培养方法,其特征在于:底部散气石(12)充气维持集卵槽(8)水体中溶氧量,保证受精卵在优良水质中达高孵化率。 According to any of a sea anemone broodstock culture method according to claim 3, wherein: the bottom of the diffuser Stone (12) to maintain the collection of eggs inflatable bath (8) the amount of dissolved oxygen in water to ensure that the fertilized egg High up in the water quality of hatching.
Description  translated from Chinese

一种海葵亲体的培养方法 Training method anemone broodstock

技术领域 Technical Field

[0001] 本发明属于水产养殖领域,特别涉及一种海葵亲体培养方法。 [0001] The present invention belongs to the field of aquaculture, particularly to a method anemone parent culture.

背景技术 Background

[0002]中华仙影海葵个体大,营养价值高,口感鲜美,是一种高档食用水产品,海葵的毒素可用于降血压、凝血,对心脏神经和肌肉有止痛作用,具有安神镇静、止痛、抗衰老、抗癌变的药用价值,中华仙影海葵也是生物毒素研究对象和观赏水族,在我国沿海有自然分布,但自然资源很少。 [0002] Chinese Xian Ying anemone individual, high nutritional value, taste delicious, it is a high-grade food fish, sea anemone toxin can be used to lower blood pressure, blood clotting, nerve and muscle of the heart analgesic effect, with calm nerves, analgesic, anti-aging, anti-cancer medicinal value change, China Xian Ying anemone also biotoxins study and ornamental aquarium, in China's coastal areas have natural distribution, but few natural resources. 目前中华仙影海葵商品完全依靠采捕自然资源,不能满足市场需求,开展人工繁殖及养殖技术的研究是水产业发展的必然途径。 Currently China Xian Ying anemone goods entirely on harvesting natural resources, can not meet the market demand, research on artificial propagation and breeding technology is the only way aquaculture development.

[0003] 进行亲体培养,使其成熟产卵受精,取得受精卵是人工繁殖的基础。 [0003] The affinity culture to mature eggs fertilized zygote is made the basis of artificial breeding. 中华仙影海葵人工繁殖的研究尚处于初创阶段,国内只有江苏省海洋水产研究所开展此项工作。 Study on China Xian Ying anemone artificial breeding is still in its infancy, there were only Jiangsu Marine Fisheries Research Institute to carry out this work. 中华仙影海葵在泥沙中营挖掘型生活,靠触手及刺细胞被动性捕食水中游过的小型水生动物为生。 Chinese Xian Ying Mining Camp anemone-type living in the sediment, tentacles and stinging cells by passive prey water swim across the small aquatic animals for a living. 人工条件下模拟自然生态采用泥沙底质培养时残饵和消化残余物不易清除,长期培养会造成底质恶化而死亡,中华仙影海葵受精卵为沉性卵,传统方法难以将受精卵与构成底质的泥沙分离,受精卵的采集成为了人工繁殖的技术瓶颈。 Simulation under artificial conditions using natural ecological culture when silt sediment and digest the bait difficult to remove the residue, sediment will cause long-term deterioration of the culture of death, China Xian Ying Shen anemone fertilized eggs, traditional methods can not be fertilized constituting separate silt sediment, the fertilized egg collecting has become a bottleneck in artificial breeding technology.

发明内容 DISCLOSURE

[0004] 本发明所要解决的问题是提供一种海葵亲体培养方法,其技术方案包括以下步骤: [0004] The present invention is to solve the problem is to provide a method anemone broodstock culture, its technical program includes the following steps:

[0005] A.亲体采集 [0005] A. broodstock collection

[0006] 采集海葵亲体,准备亲体培养; [0006] anemone broodstock collection, preparation broodstock culture;

[0007] B.建立亲体培养系统,包括: [0007] B. establishment of pro-culture systems, including:

[0008] 亲体培养槽,培养架,亲体培养罐,出水管,残饵及消化残余物分离槽,集卵网箱,集卵槽,进水管,海葵亲体,散气石,气泵及气路,水泵及管路,其中:海葵亲体培养于亲体培养罐中,亲体培养罐置于培养架中,培养架安放于亲体培养槽中,水流通过出水管流入残饵及消化残余物分离槽,受精卵随水流入集卵网箱,集卵网箱置于集卵槽中,水体经水泵及管路、进水管流入亲体培养槽,气泵及气路连接散气石,给散气石供气。 [0008] broodstock culture tank, training aircraft, broodstock culture tank, outlet pipe, bait and digestion residue separation tank, set the egg cages, egg collection tank, into the water, sea anemones broodstock, stone bulk gas, air pump and air line , pumps and pipes, including: anemone parent culture to culture broodstock tanks, broodstock culture tank placed in training aircraft, training aircraft placed in broodstock culture vessel, water flow through the outlet pipe flows into the bait tank and digested residue is separated, fertilized eggs with the water flow into the collection cages, cages placed in collection egg egg collection tank water through pumps and piping, into the water flowing into the broodstock culture tank, pump and gas connections diffuser stone to stone bulk gas supply .

[0009] 所述的一种海葵亲体培养方法,其特征在于:所述的海葵亲体是中华仙影海葵亲体。 A sea anemone broodstock culture method [0009] above, wherein: the anemone parent body is the Chinese Xian Ying anemone broodstock.

[0010] 所述的一种海葵亲体培养方法,其特征在于:步骤A采集的海葵亲体是在3-4月采集的健康、活力强、无损伤、触手齐全、足部完好的海葵亲体。 A sea anemone broodstock culture method [0010] above, wherein the steps of: A collection of sea anemones broodstock in March-April acquisition of healthy, vibrant and strong, no damage, tentacle complete, intact foot anemone broodstock.

[0011] 所述的一种海葵亲体培养方法,其特征在于:步骤B所述系统中亲体培养槽为圆形,中央底部为平缓的锥形,培养槽中央设亲体阻隔筒,中央水流通过亲体阻隔筒经中央出水口流入出水管,散气石在亲体阻隔筒内产生气升作用使亲体培养槽中形成垂直对流,进水管沿亲体培养槽上口切线方向设置,水泵及管路使进水管造成射流形成水平环流。 A sea anemone broodstock culture method [0011] above, wherein: the step system in the B broodstock culture tank is circular, the central bottom gently tapered, culture tank provided broodstock central barrier tube, central water flow through parent of a barrier through the central outlet tube flows into the outlet pipe, stone bulk gas cylinder in broodstock produce gas-lift effect blocking a parental culture grooves formed in the vertical convection, into the water along the broodstock culture catchy groove tangential direction, pumps and piping intake Water jet formation cause horizontal circulation.

[0012] 所述的一种海葵亲体培养方法,其特征在于:步骤B所述系统中还包括环形培养架模块和扇形培养架模块,其中I个环形培养架模块和多个扇形培养架模块组合成培养架整体安放于亲体培养槽内,多个亲体培养罐均匀安装于培养架上,调节水泵及管路流速保持水位。 A sea anemone broodstock culture method [0012] above, wherein: the step system B also includes an annular training aircraft modules and module training aircraft sector, where I train annular frame module and a plurality of fan-shaped module training aircraft combined into a training aircraft placed in overall broodstock culture vessel, a plurality of broodstock culture tank mounted on a uniform culture rack, adjust the flow rate of the pump and piping to maintain the water level.

[0013] 所述的一种海葵亲体培养方法,其特征在于:其中I个环形培养架模块和8个扇形培养架模块组合成培养架整体安放于亲体培养槽内,160个亲体培养罐均匀安装于培养架上。 A sea anemone broodstock culture method [0013] above, wherein: a ring which I modules and 8 training aircraft sector training aircraft modules combined into the overall training aircraft broodstock culture vessel, 160 broodstock culture evenly placed in a tank rack mounted on culture.

[0014] 所述的一种海葵亲体培养方法,其特征在于:人工投喂海葵饵料供海葵亲体摄食,残饵及消化残余物随水流进入残饵及消化残余物分离槽。 A sea anemone broodstock culture method [0014] above, wherein: artificial feeding anemone anemone broodstock diets for feeding, digestion bait and bait into the residue with water and digested residue separation tank.

[0015] 所述的一种海葵亲体培养方法,其特征在于:底部散气石充气维持集卵槽水体中溶氧量,保证受精卵在优良水质中达高孵化率。 A sea anemone broodstock culture method [0015] above, wherein: the bottom of the stone bulk gas inflator maintain dissolved oxygen in water tank set eggs to ensure high hatching rate of fertilized eggs in the water quality.

[0016] 本发明中华仙影海葵亲体培养系统有益效果: [0016] The present invention anemone China Xian Ying broodstock culture system beneficial effects:

[0017] 该系统解决受精卵收集的难题,避免了传统方法需要使用泥沙做底质,使得残饵和消化残余物易于分离去除,具有可长期培养,易于管理,受精卵连续收集,孵化率高的优点,具有良好的应用前景。 [0017] The system solves the problem of collecting fertilized eggs, avoiding the need to use the traditional method to do bottom sediment quality, making bait and digest easily separated residue removal, having a long-term culture, easy to manage, continuously collecting fertilized eggs, hatching the advantages of high, has a good prospect. 本发明的推广使用将促进海葵人工繁殖和养殖业的发展。 Promote the use of the present invention will facilitate anemone artificial breeding and the development of aquaculture.

附图说明 Brief Description

[0018] 图1为亲体培育系统侧视图 [0018] FIG. 1 is a side view of broodstock rearing system

[0019] 图2为亲体培育槽俯视图 [0019] FIG. 2 is a plan view of broodstock rearing troughs

[0020] 图3为模块结构示意图 [0020] Figure 3 is a block schematic diagram of the structure

[0021] 附图标记: I 亲体培养槽,2 培养架,3 亲体培养罐,4 亲体阻隔筒,5出水管,6残饵及消化残余物分离槽,7集卵网箱,8集卵槽,9进水管,10水位,11海葵亲体,12散气石,13气泵及气路,14 水泵及管路,15垂直对流,16中央出水口,17中央水流,18水平环流,19环形培养架模块,20扇形培养架模块。 [0021] The reference numerals: I broodstock culture tank, two training aircraft, 3 broodstock culture tanks, broodstock barrier 4 cylinder, 5 outlet pipe, 6 and digestion residue is separated bait tank, Episode 7 egg cages, the egg slots Episode 8 9 into the water, 10 water, 11 anemones broodstock, 12 stone bulk gas, air pump and air line 13, 14 pumps and piping, 15 vertical convection, 16 outlet center, 17 central water, 18 in circulation, 19 annular culture rack module, the module 20 training aircraft sector.

具体实施方式 DETAILED DESCRIPTION

[0022] 下面通过实例对本发明作进一步详细说明,但本发明不局限于以下的实例 [0022] The present invention by way of example below in further detail, but the present invention is not limited to the following examples

[0023] 实施步骤: [0023] The steps:

[0024] a 亲体采集,从自然海区采捕野生的中华仙影海葵成体,挑选健康强壮,没有破伤,触手齐全,足部完的中华仙影海葵作为亲体; [0024] a pro-picking, harvest from natural sea anemone wild Chinese Xian Ying adult, choose healthy and strong, no tetanus, tentacle complete, the foot end of the Chinese Xian Ying anemones as broodstock;

[0025] b将亲体置于亲体培养罐中培养,使用过滤海水,海水盐度20-26%。 [0025] b the parent broodstock culture tank were placed in culture, the use of filtered seawater, salinity 20-26%. ,PH7.8-8.6。 , PH7.8-8.6. 启动水栗和气栗; Start the water chestnut chestnut and gas;

[0026] c水温升高至14C以上时每日投饵,每日清除残饵和消化残余物分离槽中杂物,并清洗分离槽; [0026] c when the water temperature rises to 14 C above the daily feeding, remove bait daily and digestion residue separation tank debris, and cleaning the separator tank;

[0027] d 水温升高至24C-26C以上时,海葵亲体开始成熟产卵,每日从集卵网箱收集受精卵,取卵时避免受精卵离水。 When the [0027] d water temperature to 24 C-26 C or more, anemones begin to mature broodstock spawning, egg cage daily fertilized eggs are collected from the collector to avoid water when the fertilized ovum from.

[0028] 系统产生水流使水体在亲体培养槽一残饵及消化残余物分离槽一集卵槽一亲体培养槽之间循环。 [0028] The system generates flow of the water body in a bait tank culture of broodstock and a digestion residue separation tank egg collection trough a pro cycling culture between the grooves. 产卵后每日收集受精卵。 After the fertilized eggs were collected daily.

[0029] 系统使用直径2米的园形玻璃钢水槽作为亲体培养槽,底部为缓锥型,用无毒塑料制作培养架和培养罐,培养架采用模块化设计,便于拆装和操作管理,使用充气在阻隔筒造成气升效应使培养槽中产生垂直对流,水泵动力产生切线射流形成水平环流,使受精卵和残饵及消化残余物动态进入中央出水口,经残饵和消化残余物分离槽去除杂物,受精卵进入集卵网箱,集卵网箱采用200目筛絹作网衣,产卵季节每日从集卵网箱收集受精卵,集卵槽中海水经水泵和管路抽入亲体培养槽,水体循环使用。 [0029] The system uses two meters in diameter circular glass steel water tank as the parent body of the fermentation tank, slow conical bottom, with a non-toxic plastic produced training aircraft and tank culture, training aircraft modular design for easy disassembly and operation management, use The pneumatic cylinder caused the barrier effect of the culture vessel Airlift produce vertical convection, the pump power generating tangential jet formation horizontal circulation, so that a fertilized egg and bait and digestion residues dynamic into the central outlet, the bait and digested residue separation tank removal of debris, the fertilized egg into the egg collection cages, cage eggs set of 200 mesh silk as netting, spawning season cages to collect eggs daily collection from the fertilized egg, egg collection tank pumping seawater through the pump and piping the parent body of the fermentation tank, water recycling.

实施例 Example

[0030] 2011年4月江苏省海洋水产研究所从自然海区采捕野生的中华仙影海葵成体320头作为亲体,置于2个亲体培养槽中培养,加入过滤海水,海水盐度20-26%。 [0030] in April 2011 from Jiangsu Marine Fisheries Research Institute, Chinese wild harvesting natural sea anemone adult fairy Movies 320 as broodstock, placed two broodstock culture vessel incubation, filtered seawater, salinity 20- 26%. ,PH7.8-8.6。 , PH7.8-8.6. 水温升高至14C以上时每日投饵,每日清除残饵和消化残余物分离槽中杂物,并清洗分离槽。 When the water temperature to 14 C above the daily feeding, remove bait daily and digestion residue separation tank debris, and clean separation tank. 经过3个月培养,6月份以后水温升高至24C -26C以上时,亲体逐渐成熟开始产卵排精,每日收集集卵网箱中的受精卵用于孵化育苗1400万粒,90%以上经孵化培养成浮浪幼虫。 When after three months training, after June the water temperature to 24 C -26 C or more, began spawning broodstock mature ejaculation, daily collection set cages in egg fertilized eggs for hatching nursery 14,000,000 tablets, 90% after hatching develop into planula.

[0031] 以上仅表达了本发明的实施方式,其描述较为具体和详细,但并不能因此而理解为对发明专利范围的限制。 [0031] These are only the expression of the embodiment of the present invention, the description is more specific and detailed, but it can not therefore be construed as limiting the scope of the invention patent. 应当指出的是,对于本领域的普通技术人员来说,在不脱离本发明构思的前提下,还可以做出若干变形和改进,这些都属于本发明的保护范围。 It should be noted that those of ordinary skill in the art, in the present invention without departing from the idea of the premise, you can also make a number of modifications and improvements, which belong to the scope of the present invention. 因此,本发明专利的保护范围应以所附权利要求为准。 Accordingly, the scope of the present invention patent protection shall be subject to the appended claims.

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Referenced by
Citing PatentFiling datePublication dateApplicantTitle
CN104186395A *25 Sep 201410 Dec 2014苏州市相城区新时代特种水产养殖场Cultivation method for actiniae
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International ClassificationA01K61/00
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