CN102524116B - Artificial propagation method of Cereus sinensis - Google Patents
Artificial propagation method of Cereus sinensis Download PDFInfo
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- CN102524116B CN102524116B CN201010616805.0A CN201010616805A CN102524116B CN 102524116 B CN102524116 B CN 102524116B CN 201010616805 A CN201010616805 A CN 201010616805A CN 102524116 B CN102524116 B CN 102524116B
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- tentacle
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses an artificial propagation method of Cereus sinensis. The method comprises the following steps: capturing and selecting Cereus sinensis parents, building parent cultivation facilities, domesticating Cereus sinensis parents, collecting and hatching fertilized eggs, cultivating attached larvae and young Cereus sinensis and breeding Cereus sinensis. The method can domesticate Cereus sinensis which lives in oceans to grow and reproduce in artificial environments, achieves the purpose of artificial propagation of Cereus sinensis, and can promote development of aquaculture and development and utilization of Cereus sinensis.
Description
Technical field
The invention belongs to aquaculture field, particularly relate to Chinese celestial shadow sea anemone (
cereus sinensisverrill) artificial domestication, artificial fecundation method.
Background technology
The celestial shadow Helenopolypus coelenterate of China, base portion is bonded in silt particle and lives, and the long tubular of cylinder has barrier film system in body, mouthful coil broadly, and upper have a tentacle, to catch small-sized fish, shrimp and shellfish as food.Along The East Coast of Zhejiang And Jiangsu Provinces is regarded as first-class delicacies, mouthfeel uniqueness, and delicious flavour, is a kind of high-grade marine product.The toxin of sea anemone also can be used for hypotensive, blood coagulation, and cardiac nerve and muscle are had to analgesic effect, has the potential medical value of the calmness of calming the nerves, pain relieving, anti-ageing, anticancer change.About the research of sea anemone mainly concentrates on ecological and form biology aspect, the research of individual generation and reproductive biology aspect is few at present.Sea anemone propagate and develop the difficult problem that must first break through sea anemone artificial propagation artificially, the basis that the research of this respect and invention will become sea anemone cultivation and develop.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of simple and feasible artificial propagation method of Cereus sinensis.
Technical solution of the present invention is as follows: the artificial fecundation method of the celestial shadow sea anemone of a kind of China, comprises the following steps:
A, parent are adopted and are caught and select
Excavate from sea area to adopt and catch the celestial shadow sea anemone of the China of growing nature, select the sea anemone individuality complete without wound, vigor is strong as parent;
B, parent are raised and train
In tank, lay fine sand, establish inflation and circular flow water system in tank, control that seawater salinity is 20 ‰～26 ‰, dissolved oxygen 5mg/L is above, pH 7.8～8.6, the parent after selecting is implanted in sand and cultivated, manually bait throwing in is thrown something and fed;
C, fertilized egg are collected
The parent of raising and train is regulated and is impelled natural spawning fertilization after sexual maturity by periodicity of illumination, collects fertilized egg;
D, incubating oosperm
The fertilized egg of collecting is placed in incubation tank hatches, 24 DEG C～28 DEG C of water temperatures, salinity 20 ‰～26 ‰, and inflation hatching, changes water 50% every day;
E, Metamorphore are cultivated and spatfall
The Metamorphore that hatching obtains is placed in the tank of growing seedlings and cultivates, and inflation, changes water 50%～100% every day, and the particle diameter 50 μ m～80 μ m bait of throwing something and feeding are cultivated to eight former bases appearance of tentacle, drop into Seedling culturing device spatfall;
F, young certain herbaceous plants with big flowers are cultivated
The Seedling culturing device of attached good seedling is moved in young certain herbaceous plants with big flowers cultivation pond and cultivated, 8 tentacle～16 tentacle children certain herbaceous plants with big flowers are thrown artemia nauplii and make bait, 16 tentacle～24 tentacle children certain herbaceous plants with big flowers throwing artemia naupliis, copepoda, halogen worm adult, oppossum shrimp make bait, and 24 tentacle～48 tentacles throw oppossum shrimps and fishes and shrimps meat is made bait;
G, sea anemone cultivation
Children certain herbaceous plants with big flowers is cultured to and is peeled off Seedling culturing device after 24 tentacle～48 tentacle sea anemones and move in culturing pool and cultivate, and condition of culture and method and parent are raised and train identical.
Described in step b, to raise and train be in tank, to lay the thick fine sand of 40cm to parent, enters seawater to 20cm on Shamian Island.
Described in step b parent raise and train be will be described parent implant in sand and cultivate, cultivate 20/m of density
2～50/m
2, artificial bait throwing in when cultivation water temperature rises to more than 14 DEG C, is cut into 5cm by fish, shrimp, shellfish meat
3size is thrown something and fed.
Described in steps d, incubating oosperm is fertilized egg to be placed in to incubation tank hatch, hatching density 5 × 10
6grain/m
3～10 × 10
6grain/m
3.
Described in steps d, incubating oosperm adds 4 × 10 in seawater
-6ton/cubic meter～8 × 10
-6the disodium ethylene diamine tetraacetate of ton/cubic meter concentration.
Described in step e, Metamorphore cultivation and spatfall are Metamorphore to be placed in to the tank of growing seedlings cultivate, and cultivate density 3 × 10
6head/m
3～5 × 10
6head/m
3, the particle diameter 50 μ m～80 μ m bait of throwing something and feeding.
Described in step e, Metamorphore cultivation and spatfall are dropped into Seedling culturing device spatfall, 100/m of spatfall density in cultivation in the time that eight former bases of tentacle occur
2～500/m
2.
Beneficial effect of the present invention: the present invention by adopting of the celestial shadow sea anemone parent of natural waters China catch, raise and train, hatching, seedling growing process, creating sea anemone raises and train and culture facility, make the sea anemone domestication of perching life originally in ocean adapt to cultivate under artificial environment and ripe breeding, solve the technical barrier of sea anemone artificial propagation, successfully carry out the artificial breeding of sea anemone, sea anemone is raised and train and artificial fecundation method practicability and effectiveness.The present invention deepens sea anemone biology and ecological study and carries out the basis that sea anemone is numerous, cultivate, and of the present invention promoting the use of will promote the development of culture fishery and the exploitation of sea anemone.
embodiment
Below by embodiment, the present invention is further detailed explanation, but the present invention is not limited to following embodiment.
An artificial fecundation method for the celestial shadow sea anemone of China, comprises the following steps:
A, parent are adopted and are caught and select
Excavate from sea area to adopt and catch the celestial shadow sea anemone of the China of growing nature, select the sea anemone individuality complete without wound, vigor is strong as parent;
B, parent are raised and train
In tank, lay thick fine sand, establish inflation and circular flow water system in tank, seawater salinity is 20～26, dissolved oxygen 5mg/L is above, pH 7.8～8.6, and the parent after selecting is implanted in sand and cultivated, and manually bait throwing in is thrown something and fed;
C, fertilized egg are collected
The parent of raising and train is regulated and is impelled natural spawning fertilization after sexual maturity by periodicity of illumination, collects fertilized egg;
D, incubating oosperm
The fertilized egg of collecting is placed in incubation tank hatches, 24 DEG C～28 DEG C of water temperatures, salinity 20～26, and inflation hatching, changes water 50% every day;
E, Metamorphore are cultivated and spatfall
The Metamorphore that hatching obtains is placed in the tank of growing seedlings and cultivates, and inflation is cultivated, and changes water 50%～100% every day, and the particle diameter 50 μ m～80 μ m bait of throwing something and feeding are cultivated to eight former bases appearance of tentacle, drop into Seedling culturing device spatfall;
F, young certain herbaceous plants with big flowers are cultivated
The Seedling culturing device of attached good seedling is moved in young certain herbaceous plants with big flowers cultivation pond and cultivated, 8 tentacle～16 tentacle children certain herbaceous plants with big flowers are thrown artemia nauplii and make bait, 16 tentacle～24 tentacle children certain herbaceous plants with big flowers throwing artemia naupliis, copepoda, halogen worm adult, oppossum shrimp make bait, and 24 tentacle～48 tentacles throw oppossum shrimps and fishes and shrimps meat is made bait;
G, sea anemone cultivation
Children certain herbaceous plants with big flowers is cultured to and is peeled off Seedling culturing device after 24 tentacle～48 tentacle sea anemones and move in culturing pool and cultivate, and condition of culture and method and parent are raised and train identical.
Described in step b, to raise and train be in tank, to lay the thick fine sand of 40cm to parent, enters seawater to 20cm on Shamian Island.Described in step b parent raise and train be will be described parent implant in sand and cultivate, cultivate 20/m of density
2～50/m
2, artificial bait throwing in when cultivation water temperature rises to more than 14 DEG C, is cut into 5cm by fish, shrimp, shellfish meat
3size is thrown something and fed.Described in steps d, incubating oosperm is fertilized egg to be placed in to incubation tank hatch, hatching density 5 × 10
6grain/m
3～10 × 10
6grain/m
3.Described in steps d, incubating oosperm adds 4 × 10 in filtering sea
-6ton/cubic meter～8 × 10
-6the disodium ethylene diamine tetraacetate of ton/cubic meter concentration.Described in step e, Metamorphore cultivation and spatfall are Metamorphore to be placed in to the tank of growing seedlings cultivate, and cultivate density 3 × 10
6head/m
3～5 × 10
6head/m
3, the particle diameter 50 μ m～80 μ m bait of throwing something and feeding.Described in step e, Metamorphore cultivation and spatfall are dropped into Seedling culturing device spatfall, 100/m of spatfall density in cultivation in the time that eight former bases of tentacle occur
2～500/m
2.
embodiment
1, sea anemone is raised and train
(1) sea area is adopted and is caught the celestial shadow sea anemone of the China of growing under nature.Select the sea anemone complete without wound, vigor is strong as parent
(2) in tank, lay the thick fine sand of 40cm, in tank, establish inflation and circular flow water system, enter seawater to 20cm on Shamian Island, seawater salinity 20 ‰～26 ‰, more than maintenance dissolved oxygen 5mg/L, pH 7.8～8.6.Sea anemone parent is implanted in sand and cultivated, cultivate 20/m of density
2～50/m
2.Artificial bait throwing in when cultivation water temperature rises to more than 14 DEG C, is cut into 5cm by fish, shrimp, shellfish meat
3size is thrown something and fed
2, sea anemone artificial propagation
(1) regulate natural spawning fertilization after the China's celestial shadow sea anemone sexual maturity that impels cultivation by periodicity of illumination, utilize fertilized egg automatic collecting device, collect fertilized egg every day.Fertilized egg is placed in incubation tank hatches, hatching density 5 × 10
6grain/m
3～10 × 10
6grain/m
3, 24 DEG C～28 DEG C of water temperatures, salinity 20～26, inflation hatching, changes water 50% every day.
(2) incubating oosperm obtains Metamorphore, Metamorphore is placed in to the tank of growing seedlings and cultivates, and cultivates density 3 × 10
6head/m
3～5 × 10
6head/m
3, inflation is cultivated, and day is changed water 50%～100%, the particle diameter 50 μ m～80 μ m bait of throwing something and feeding.Cultivate to eight former bases appearance of tentacle, drop into Seedling culturing device spatfall, 100/m of spatfall density
2～500/m
2.
(3) Seedling culturing device of attached good seedling moves into young certain herbaceous plants with big flowers and cultivates cultivation in pond, and 8 tentacle～16 tentacle children certain herbaceous plants with big flowers are thrown artemia naupliis and make bait, and 16 tentacle～24 tentacle children certain herbaceous plants with big flowers are thrown artemia nauplii or copepoda, and arrange in pairs or groups halogen worm adult or oppossum shrimp make bait.24 tentacle～48 tentacles throw oppossum shrimp and fishes and shrimps meat is made bait.Children sea anemone is cultivated the Seedling culturing device of peeling off of 24 tentacle～48 tentacles, moves into the seed as sea anemone cultivation in culturing pool.
Below only expressed embodiments of the present invention, it describes comparatively concrete and detailed, but can not therefore be construed as limiting the scope of the patent.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (1)
1. an artificial fecundation method for the celestial shadow sea anemone of China, comprises the following steps:
A, parent are adopted and are caught and select
Excavate from sea area to adopt and catch the celestial shadow sea anemone of the China of growing nature, select the sea anemone individuality complete without wound, vigor is strong as parent;
B, parent are raised and train
In tank, lay fine sand, establish inflation and circular flow water system in tank, seawater salinity is 20 ‰～26 ‰, dissolved oxygen 5mg/L is above, pH 7.8～8.6, the parent after selecting is implanted in sand and is cultivated, manually bait throwing in;
C, fertilized egg are collected
The parent of raising and train is regulated and is impelled natural spawning fertilization after sexual maturity by periodicity of illumination, collects fertilized egg;
D, incubating oosperm
The fertilized egg of collecting is placed in incubation tank hatches, 24 DEG C～28 DEG C of water temperatures, salinity 20 ‰～26 ‰, and inflation hatching, changes water 50% every day;
E, Metamorphore are cultivated and spatfall
The Metamorphore that hatching obtains is placed in the tank of growing seedlings and cultivates, and inflation, changes water 50%～100% every day, and the particle diameter 50 μ m～80 μ m bait of throwing something and feeding are cultivated to eight former bases appearance of tentacle, drop into Seedling culturing device spatfall;
F, young certain herbaceous plants with big flowers are cultivated
The Seedling culturing device of attached good seedling is moved in young certain herbaceous plants with big flowers cultivation pond and cultivated, 8 tentacle～16 tentacle children certain herbaceous plants with big flowers are thrown artemia nauplii and make bait, 16 tentacle～24 tentacle children certain herbaceous plants with big flowers throwing artemia naupliis, copepoda, halogen worm adult, oppossum shrimp make bait, and 24～48 tentacles children certain herbaceous plants with big flowers throw oppossum shrimps and fishes and shrimps meat is made bait;
G, sea anemone cultivation
Children certain herbaceous plants with big flowers is cultured to and is peeled off Seedling culturing device after 24 tentacle～48 tentacle sea anemones and move in culturing pool and cultivate, and condition of culture and method and parent are raised and train identical.
2
.according to claim 1, an artificial fecundation method for the celestial shadow sea anemone of China, is characterized in that: described in step b, to raise and train be in tank, to lay the thick fine sand of 40cm to parent, enters filtering sea to 20cm on Shamian Island.
3
.according to claim 1, an artificial fecundation method for the celestial shadow sea anemone of China, is characterized in that: described in step b parent raise and train be will be described parent implant in sand and cultivate, cultivate 20/m of density
2～50/m
2, artificial bait throwing in when cultivation water temperature rises to more than 14 DEG C, is cut into 5cm by fish, shrimp, shellfish meat
3size is thrown something and fed.
4
.according to claim 1, an artificial fecundation method for the celestial shadow sea anemone of China, is characterized in that: described in steps d, incubating oosperm is fertilized egg to be placed in to incubation tank hatch, hatching density 5 × 10
6grain/m
3～10 × 10
6grain/m
3.
5
.according to claim 1, an artificial fecundation method for the celestial shadow sea anemone of China, is characterized in that: described in steps d, incubating oosperm adds 4 × 10 in filtering sea
-6ton/cubic meter～8 × 10
-6the disodium ethylene diamine tetraacetate of ton/cubic meter concentration.
6
.according to claim 1, an artificial fecundation method for the celestial shadow sea anemone of China, is characterized in that: described in step e, Metamorphore cultivation and spatfall are Metamorphore to be placed in to the tank of growing seedlings cultivate, and cultivate density 3 × 10
6head/m
3～5 × 10
6head/m
3, the particle diameter 50 μ m～80 μ m bait of throwing something and feeding.
7
.according to claim 1, an artificial fecundation method for the celestial shadow sea anemone of China, is characterized in that: described in step e, Metamorphore cultivation and spatfall are dropped into Seedling culturing device spatfall, 100/m of spatfall density in cultivation in the time that eight former bases of tentacle occur
2～500/m
2.
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| CN102524116B true CN102524116B (en) | 2014-11-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186395A (en) * | 2014-09-25 | 2014-12-10 | 苏州市相城区新时代特种水产养殖场 | Cultivation method for actiniae |
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| CN103205350A (en) * | 2012-12-21 | 2013-07-17 | 江苏省海洋水产研究所 | Preparation method of sea anemone wine |
| CN105684969B (en) * | 2016-02-03 | 2019-09-20 | 浙江省海洋水产研究所 | The cultivation apparatus and cultural method of sea anemone |
| CN106386579A (en) * | 2016-08-31 | 2017-02-15 | 山东东方海洋科技股份有限公司 | Method for sea anemone culture in pond |
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| CN1417333A (en) * | 2001-11-06 | 2003-05-14 | 北京博奥环宇生物技术有限公司 | Cloning, Expression and bioactivity of new actinion toxin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104186395A (en) * | 2014-09-25 | 2014-12-10 | 苏州市相城区新时代特种水产养殖场 | Cultivation method for actiniae |
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| CN102524116A (en) | 2012-07-04 |
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