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Neisseria gonorrhoeae-praktikum mikrobiologi.pptx
- 2. Gonorrhea : Neisseria gonorrhoeae 2 • Termasuk dlm famili Neisseriaceae • Morfologi Coccus Gram Θ (0.6 x 1.0 m) sering ‘diplococcus’ (seperti biji kopi) Tak mempunyai flagela Tak berspora Mempunyai pili (strain virulen)
- 3. Neisseria Associated Diseases (ophthalmia neonatorum)
- 4. Epidemiology of Gonorrhea • Sexually-transmitted disease • Found only in humans • Asymptomatic carriage is major reservoir • Lack of protective immunity and therefore reinfection, partly due to antigenic diversity of strains • Higher risk of disseminated disease in patients with complement deficiencies
- 5. In MEN Urethritis; Epididymitis Most infections among men are acute and symptomatic with purulent discharge & dysuria (painful urination) after 2-5 day incubation period The two bacterial agents primarily responsible for urethritis among men are N. gonorrhoeae and Chlamydia trachomatis Differences Between Men & Women with Gonorrhea
- 6. Differences Between Men & Women with Gonorrhea • In WOMEN • Cervicitis; Vaginitis; Pelvic Inflammatory Disease (PID); Disseminated Gonococcal Infection (DGI) • Women often asymptomatic or have atypical indications; Often untreated until PID complications develop • Pelvic Inflammatory Disease (PID) • May also be asymptomatic, but difficult diagnosis accounts for many false negatives • Can cause scarring of fallopian tubes leading to infertility or ectopic pregnancy
- 7. Gonorrhea Females Males 50% risk of infection after single exposure 20% risk of infection after single exposure Asymptomatic infections frequently not diagnosed Most initially symptomatic (95% acute) Major reservoir is asymptomatic carriage in females Major reservoir is asymptomatic carriage in females Genital infection include cervix (cervicitis), but vagina, urethra, rectum can be colonized Genital infection generally restricted to urethra (urethritis) with purulent discharge and dysuria Ascending infections in 10-20% including salpingitis, tubo-ovarian abscesses, pelvic inflammatory disease (PID) , can lead to sterility Rare complications may include epididymitis, prostatitis, and periurethral abscesses Disseminated infections more common, including septicemia, infection of skin and joints (1-3%) Disseminated infections are very rare Can infect infant at delivery (conjunctivitis, opthalmia neonatorum) More common in homosexual men
- 8. • Fisiologi Sifat biokimia & ciri kultur : - aerob - oksidase +, katalase +, - sangat peka terhadap lingkungan merugikan : drying, chilling, pH, sinar matahari 8 - Kultur : pada 3-10% CO2 medium selektif : Thayer Martin Agar (Chocolate Agar + Vancomycin, Colistin, Nystatin) Identifikasi : metabolisme glukosa : + metabolisme maltosa, sukrosa, laktosa : -
- 9. Struktur Antigen - Ag pilus - Protein pada outer membrane - Lipo oligosakarida pada outer membrane 9 Determinan Patogenesis Pili : faktor virulensi Komponen pada outer membrane Peptidoglycan Ig A protease (hanya pada Neisseria pathogen)
- 10. • Patogenesis Infeksi berawal pada epitel kolumnar urethra, saluran periurethral dan kelenjar kelamin kuman melekat pada permukaan sel (dengan pili) penetrasi ke subepitel (hari III) respon peradangan oleh PMN (obstruksi oleh eksudat) Penyebaran sering terjadi lewat pembuluh limfe, dapat pula lewat pembuluh darah 10 • Resistensi - Non spesifik (wanita : hormon & pH genital ~ siklus haid; pria : pH, osmolaritas, kadar urea dari urin ) - Spesifik ( sistem imun humoral terutama Ig A & Ig G, Complement)
- 11. Pemeriksaan Laboratorik Bahan Pemeriksaan Nanah/sekret diambil dari uretra, servix, prostat, mukosa tenggorok, kadang-kadang cairan synovia Pemeriksaan Mikroskopik Spesimen yang diambil dibuat preparat oles dan dilakukan pengecatan gram. Gambaran mikroskopik dari Neisseria gonorrhoe adalah pada proses akut tampak diplokokkus gram negatif intraseluler dalam sel-sel darah putih dengan pengecatan gram Pemeriksaan makroskopik (Kultur/penanaman) • Sekret diambil dari uretra (pada laki-laki) dan cervix (pada wanita) kemudian digores pada media Thayer Martin (media selektif yang diperkaya) dan diinkubasi dalam atmosfer yang mengandung CO2 5% (metode lilin padam) pada 350 – 370 Cselama 48 jam. Untuk menghindari kontaminasi, media diberi antimikroba.
- 12. SPESIMEN
- 13. Diplococcus gram negatif • Diplokokkus gramnegative • Biji kopi/ginjal berhadapan (bagian tengah rata/cekung) • Non motil,non spora • ø 0,8 mm
- 14. neisseria gonorrhoeae thayer martin agar Koloni pada Media Thayer Martin: Ciri makroskopis Neisseriae go : Koloni mukoid, cembung, mengkilat, menonjol, tidak mempunyai pigmen, transparan, non hemolitik, oksidase (+), obligat aerob, memfermentasi KH (Glukosa) dan tumbuh pada CO2 (5 – 10%).
- 16. Tugas mahasiswa 1. Pengamatan 2. Diskusi kasus (buat kasus scenario untuk diskusi)